Plant pathogen testing: Yeasts and Molds
In-house testing for Yeasts and Molds
HSG-AME's Yeasts and Molds PCR assay is employed to detect and quantify the amount of fungal growth on plant material, and allow for identification of viable yeast and mold species present.
Mold is a type of fungus that grows in multicellular filaments called hyphae. These tubular branches have multiple, genetically identical nuclei, yet form a single organism, known as a colony. In contrast, yeast is a type of fungus that grows as a single cell.
Traditional methods require dichloran rose bengal chloramphenicol agar, oxytetracycline glucose yeast extract agar and rose bengal chloramphenicol agar to enumerate of yeasts and molds.
PCR testing rapidly determines presence/absence without long incubation periods.
Yeasts can cause infection in people with compromised immune systems. They can cause the disease cryptococcossis, which is found in 7-9% of AID patients in the US. The yeast candida can also cause candidiasis in humans.
The HSG/AME assay is certified as valid. It has been tested using recognized FDA BAM methods and ISO/AWI TS 16099: Water quality — Polymerase chain reaction (PCR) for the detection and quantification of microorganisms — Quality control and validation of molecular methods, and SO 20836:2021--Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of microorganisms — Thermal performance testing of thermal cyclers.
For further information, contact:
Andy Moreno, PhD
Bacterial Surveillance Systems Engineer
AME Certified Laboratories
650-445-4115
Plant pathogen testing: Pythium pcr assay
In-house testing for Pythium is available for indoor growers
Pythium is a genus of parasitic oomycetes, sometimes referred to as Pythium root rot or water mold and is common in lettuce and other leafy green products.
This pathogen is particularly hazardous for indoor growers and controlled environment agriculture (CEA) producers .
Effective disease management is hindered by the lack of fast, accurate pathogen detection methods and tools.
HSG-AME can equip your in-house laboratory, train your team to run the tests, provide your DNA test kits, and certify the lab and the technicians to surveil your indoor grow facility for the Pythium plant pathogen.
- Sample collection protocols (water, soil, plant materials)
- Sample preparation methods
- DNA purification protocol
- Sample testing kits
- Test results interpretation and decision-making
- All test kits will be validated for analytically verification for microbial inclusivity, microbial exclusivity, and nominal Limit of Detection (LoD) of below 100 genomic copies per reaction
- Assay will not detect DNA from other potential commensal bacterial/fungal contaminants found in plant/water samples
- Sample volume input of less than or equal to 5 uL
- Fixed price per test for each sample examined by DNA analysis
- Test turnaround time about one (1) hour from sample preparation
- Each kit includes a Positive Control template for each run validation
HSG-AME's real-time PCR tool is valuable for the early surveillance and detection of Pythium spread in growing facilities worldwide.
The HSG/AME assay is certified as valid. It has been tested using recognized FDA BAM methods and ISO/AWI TS 16099: Water quality — Polymerase chain reaction (PCR) for the detection and quantification of microorganisms — Quality control and validation of molecular methods, and SO 20836:2021--Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of microorganisms — Thermal performance testing of thermal cyclers.
For further information, contact:
Andy Moreno, PhD
Bacterial Surveillance Systems Engineer
AME Certified Laboratories
650-445-4115
New Plant Pathogen Kits
New kits available for growers
Quantitative, real-time polymerase chain reaction (qRT-PCR) kits for the following plant pathogens:
--Pseudomonas
--Fusarium
--Rhizoctonia
Sample preparation, test preparation, test specifications
Answers to these questions are available upon request.